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Three Dimension (3D) Printing is a technology for printing a material by producing a three-dimensional object using the Additive Manufacturing technique, with the layer manufacturing method. In this study, printing three-dimensional objects using materials made from polylactic acid. Polylactic acid is a type of plastic filament material that is biodegradable, more easily decomposed than other plastic filaments, so it is environmentally friendly. This research discusses about the printing and testing of materials made from polylactic acid with room temperature variations of 18⁰C, 25⁰C, 30⁰C. Tests were carried out to determine the effect of room temperature on the honeycomb structure by conducting a tensile test and a bending test. Printouts at room temperature variations of 18⁰C have a higher average stress value in the bending test and have a higher average Young's modulus value in the tensile test compared to 25⁰C and 30⁰C. Based on the results of the bending test at 18⁰C it has an average stress value of 0.000126 MPa, for the tensile test at 18⁰C it has an average Young's modulus value of 1.13827MPa.